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KMID : 0378019670100050089
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New Medical Journal
1967 Volume.10 No. 5 p.89 ~ p.95
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Autoradiographic studies on the effect of sexual hormone on DNA synthesis in mice colonic epithelium using H^(3)-thymidine
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Abstract
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Effects of sexual hormones on the rate of cell division in the colonic epithelium of young adult mice of both sexes, weighing (about 20 grams each, were studied by autoradiographic
technique. 15 male mice were evenly divided into three groups: a normal control group, another group which was castrated and the third group administered with testosterone propeionate after castration. 20 female mice were also evenly divided into four groups: one group in diestrus, another group in estrus, a third group which was cstrated and the fourth group administered with estradiol benzoate after castration.
The sexual hormones were injected subcutanously into the castrated mice twice at an interval of over 24 hours with a does of 2pgm/gm body weight.
24 hours after the hormone injection, the animals were given intravenously a does´ of 0.8 pc/gm body weight of tritiated thymidine and were sacrificed seven hours thereafter. Pieces of the colon were fixed in Bouin´s fluid and made into paraffin sections of 3,u.
Labelled nuclei per 1000 nuclei in the colonic glandular epithelium were calculated and the ratio was tentativly set as the radioactive index.
1. The radioactive indices of the colonic epithelium of the castrated mice were markedly reduced compared with those of the normal group and the indices were restored to the nor-
mal or, a little higher level after hormone injection.
2. During diestrus period, the radioactive indices of the colonic epithelial cells in the female mice` were generally larger than those in the estrus period.
Consequently, it is to be believed that the injected sexual hormones increase the value of DNA synthesis, with the result that renewal rate of the colonic crypt cells of the castrated ´mice is accelerated, finally the normal or a little higher level is reached.
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